Roche has expanded its partnership with Bio-Techne by offering drug discovery researchers in the U.S. new chromogenic detection kits for automated in situ hybridization (ISH) tissue analysis.
The new Roche kits include the DISCOVERY mRNA Purple HRP detection kit (RUO) and the DISCOVERY mRNA Teal HRP detection kit (RUO). The purple and teal detection kits feature more translucent dyes that are ideal for co-localization studies for mRNA ISH as well as the ability to perform immunohistochemistry (IHC) and ISH for protein-RNA co-detection on the same slide. Both kits are designed to be used on the Roche DISCOVERY ULTRA staining platform alongside the recently released Advanced Cell Diagnostics (ACD) RNAscope VS Universal HRP Reagent Kit, which offers improved signal-to-noise ratio and robustness.
In addition, Roche's mRNA Duplex AMP kit (RUO) now has an expanded application that enables the new mRNA Teal HRP detection kit to be used with Roche's existing mRNA RED detection kit (RUO) for dual mRNA ISH detection. The teal/red combination provides a distinct visual contrast and the translucency of the teal dye allows for studying co-expression of mRNA ISH targets, where the resulting teal and red dyes overlap to produce an indigo color. This new application is intended to be used with the recently released RNAscope VS Duplex Reagent Kit, which delivers simultaneous in situ detection of two mRNA species.
"Expanding our partnership with Bio-Techne enables Roche to offer scientists a broader suite of tools to expand their tissue research capabilities," said Holly Egan, vice president of marketing for tissue diagnostics at Roche Diagnostics Corporation. "Giving researchers the opportunity to automate these assays on a Roche platform helps them save time and generate data faster to accelerate drug discovery research."
The new detection kits and dual mRNA ISH application enable researchers to increase their multiplexing possibilities, optimize staining conditions for challenging antibodies and probes, easily run complex assays, conserve tissue samples and save time.
"We're pleased to partner with Roche to provide researchers access to a fully automated, optimized solution for the performance of RNAscope, our highly sensitive and specific RNA ISH technology for the detection of any RNA in any species," said Kim Kelderman, president of Diagnostics and Genomics at Bio-Techne Corporation. "The expanded chromogenic detection options on the Roche platform provide our customers with exceptional visualization and flexibility for RNAscope staining and ISH-IHC co-staining."
Widely used in cancer research, ISH and IHC tissue-staining methods provide information that helps researchers better understand mechanisms of disease and accelerate drug development. Automating ISH and IHC on the DISCOVERY ULTRA platform enables scientists to generate standardized, reproducible results and eliminate over 90 percent of manual processes so they can spend more time focusing on their research.
The mRNA ISH detection kits from Roche are intended for research use only on the DISCOVERY ULTRA platform with the ACD RNAscope reagent kits from Bio-Techne.