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Driving Diabetes-Related Research Through Pathway Dissection of Human iPSC-Derived Cell Models

Coby Carlson, PhD
Tuesday, February 16, 2016
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Chapters

  • Introduction
  • Similar Tutorial Last Year at SLAS 2015
  • Transformative Potential of iPSC Technology
  • Multi-mode Microplate Reader
  • Cisbio has Pioneered and Refined Cellular TR-FRET Assays
  • Previous Success: iPSC-based Alzheimer’s Disease Model
  • Workflow: Two-plate Assay Protocol
  • Analysis of HTRF Data – Many Ways to Slice It!
  • Disease Modeling Approaches Using iPS Cells
  • Diabetes (T2D) and Insulin Resistance (IR)
  • Insulin Decreases Glucose Production in iCell Hepatocytes
  • Modulating Insulin Signaling in iCell Hepatocytes
  • Glucagon-mediated Cell Signaling Events
  • Pro-inflammatory Cytokine Signaling in iCell HC 2.0
  • Typical Cell Models for Skeletal Muscle
  • Insulin Stimulates Glucose Uptake in iCell Skeletal Myoblasts
  • Dissecting Cell Signaling Events in iCell Skeletal Myoblasts
  • How to Model Disease in a Cellular System?
  • Induced Disease Model for Insulin Resistance (a start)
  • Summary
  • Mechanism for Crosstalk between T2D and AD Identified
  • Custom Assay Solutions
  • THANK YOU !!
 

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