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Driving Diabetes-Related Research Through Pathway Dissection of Human iPSC-Derived Cell Models
Coby Carlson, PhD
Tuesday, February 16, 2016
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Chapters
Introduction
Similar Tutorial Last Year at SLAS 2015
Transformative Potential of iPSC Technology
Multi-mode Microplate Reader
Cisbio has Pioneered and Refined Cellular TR-FRET Assays
Previous Success: iPSC-based Alzheimer’s Disease Model
Workflow: Two-plate Assay Protocol
Analysis of HTRF Data – Many Ways to Slice It!
Disease Modeling Approaches Using iPS Cells
Diabetes (T2D) and Insulin Resistance (IR)
Insulin Decreases Glucose Production in iCell Hepatocytes
Modulating Insulin Signaling in iCell Hepatocytes
Glucagon-mediated Cell Signaling Events
Pro-inflammatory Cytokine Signaling in iCell HC 2.0
Typical Cell Models for Skeletal Muscle
Insulin Stimulates Glucose Uptake in iCell Skeletal Myoblasts
Dissecting Cell Signaling Events in iCell Skeletal Myoblasts
How to Model Disease in a Cellular System?
Induced Disease Model for Insulin Resistance (a start)
Summary
Mechanism for Crosstalk between T2D and AD Identified
Custom Assay Solutions
THANK YOU !!
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